Evaluation of cell biomarkers as in vitrophotoprotective assays for sunscreen formulations

Objectives: The aim of this study was to evaluate cellular indicators, which change with exposure ultraviolet (UV) radiation and can be used as parameters for measuring sunscreens efficiency. Methods: Commercial strains L929 HaCaT cells (skin dermis epidermis, respectively), from the cell bank Rio de Janeiro, were exposed different doses UVA (350 nm) UVB (309 radiation. evaluation photoprotective potential analyzed viability, lipid peroxidation ROS generation tests. Samples sunscreen SPF values ranging 15 60 applied a quartz plate superimposed on top microplate containing culture, then system irradiated. Results: viability two lines remained unchanged after When radiation, reduction in increase peroxides dose-dependent, that is, they varied 3.15% 95.4%, 1.2 42.7 nM MDA/pg protein, respectively, both strain. dose 0.5 J/cm2 reduced by 41.4%±1.67 number viable cells, 30 promoted oxidation protein. These selected effectiveness commercial sunscreens. Sunscreens rays could prevent loss (viability around 100% higher SPF) formation (30 80% peroxide levels). None strains, submitted formed amounts intracellular dose-dependent manner. Under only line produced largest After treating these formulations (20 J/cm2), researchers observed amount formed. Conclusions: promising capacity against expressed strain discriminate ROS. results suggest vitro tests damage caused predict indicators contribute minimize initial phase product research development.